Emotional Exhaustion Among Child Welfare Social Workers in Southern California: A Qualitative Study

The purpose of this study was to explore emotional exhaustion among child welfare social workers in Southern California. Most of the research studies on this area have been conducted outside the southern region of the Golden State. Through semi-structured interviews with 12 current and former child welfare social workers, this study found three major themes: (1) child welfare social workers suffer from a multifaceted form of emotional exhaustion; (2) child welfare social workers believe that the benefits of child welfare ultimately outweigh its costs, and (3) child welfare social workers use a variety of ways to cope with work-related emotional exhaustion. Implications of these findings for child welfare are discussed. Keywords: emotional exhaustion, child welfare, social work, qualitative research methodolog

Modeling and Control of a <i>DC-DC</i> Buck–Boost Converter with Non-Linear Power Inductor Operating in Saturation Region Considering Electrical Losses

The present work proposes a nonlinear model of a buck–boost DC-DC power converter considering the nonlinear magnetic characteristics of the power inductor and electrical losses of the system. The Euler–Lagrange formalism is used for formulating the proposed model. Previous research works have reported mathematical models to describe power inductor dynamics. However, a gap in the literature remains regarding modeling this kind of element when it operates within power converters. Also, a linear-based controller scheme is proposed to regulate a non-ideal buck–boost DC-DC power converter. A methodology for tuning the proposed controller is presented, which considers the nonlinear model structure of the power converter, the linearization procedure based on an identification process, and a frequency domain analysis based on the approximated linear model. Finally, the tuned control scheme is tested on the nonlinear model of the power converter under several operational conditions showing excellent performance by effectively regulating the output voltage. The results are compared with those derived from alternative control strategies, and a better performance is generally obtained

Perfluorooctane Sulfonate (PFOS) and Perfluorohexane Sulfonate (PFHxS) Alters Protein Phosphorylation, Increase ROS Levels and DNA Fragmentation during In Vitro Capacitation of Boar Spermatozoa

17 Pág.Perfluorooctane sulfonate (PFOS) and perfluorohexane sulfonate (PFHxS) are toxic and bioaccumulative, included in the Stockholm Convention's list as persistent organic pollutants. Due to their toxicity, worldwide distribution, and lack of information in spermatozoa physiology during pre-fertilization processes, the present study seeks to analyze the toxic effects and possible alterations caused by the presence of these compounds in boar sperm during the in vitro capacitation. The spermatozoa capacitation was performed in supplemented TALP-Hepes media and mean lethal concentration values of 460.55 μM for PFOS, and 1930.60 μM for PFHxS were obtained. Results by chlortetracycline staining showed that intracellular Ca2+ patterns bound to membrane proteins were scarcely affected by PFOS. The spontaneous acrosome reaction determined by FITC-PNA was significantly reduced by PFOS and slightly increased by PFHxS. Both toxic compounds significantly alter the normal capacitation process from 30 min of exposure. An increase in ROS production was observed by flow cytometry and considerable DNA fragmentation by the comet assay. The immunocytochemistry showed a decrease of tyrosine phosphorylation in proteins of the equatorial and acrosomal zone of the spermatozoa head. In conclusion, PFOS and PFHxS have toxic effects on the sperm, causing mortality and altering vital parameters for proper sperm capacitation.This research was funded by CONACyT, Mexico fellowships 283833 to I.O.-L., 593020 to P.B.O.-S., 940985 to O.M.-P.; Ramón y Cajal contract from the Spanish Ministry of Science and Innovation (RYC-2016-20147) to S.P.-C. This study was partially supported by CONACyT, Mexico, Grants 52877-Z/66953 and 0105961, and Spain, Grant RTI2018-096736-A-I00 from the Spanish Ministry of Science and Innovation.Peer reviewe

Perfluorooctane Sulfonate (PFOS) and Perfluorohexane Sulfonate (PFHxS) Alters Protein Phosphorylation, Increase ROS Levels and DNA Fragmentation during In Vitro Capacitation of Boar Spermatozoa

Perfluorooctane sulfonate (PFOS) and perfluorohexane sulfonate (PFHxS) are toxic and bioaccumulative, included in the Stockholm Convention&rsquo;s list as persistent organic pollutants. Due to their toxicity, worldwide distribution, and lack of information in spermatozoa physiology during pre-fertilization processes, the present study seeks to analyze the toxic effects and possible alterations caused by the presence of these compounds in boar sperm during the in vitro capacitation. The spermatozoa capacitation was performed in supplemented TALP-Hepes media and mean lethal concentration values of 460.55 &mu;M for PFOS, and 1930.60 &mu;M for PFHxS were obtained. Results by chlortetracycline staining showed that intracellular Ca2+ patterns bound to membrane proteins were scarcely affected by PFOS. The spontaneous acrosome reaction determined by FITC-PNA was significantly reduced by PFOS and slightly increased by PFHxS. Both toxic compounds significantly alter the normal capacitation process from 30 min of exposure. An increase in ROS production was observed by flow cytometry and considerable DNA fragmentation by the comet assay. The immunocytochemistry showed a decrease of tyrosine phosphorylation in proteins of the equatorial and acrosomal zone of the spermatozoa head. In conclusion, PFOS and PFHxS have toxic effects on the sperm, causing mortality and altering vital parameters for proper sperm capacitation

RegulonDB version 9.0: high-level integration of gene regulation, coexpression, motif clustering and beyond

International audienceRegulonDB (http://regulondb.ccg.unam.mx) is one of the most useful and important resources on bacterial gene regulation, as it integrates the scattered scientific knowledge of the best-characterized organism, Escherichia coli K-12, in a database that organizes large amounts of data. Its electronic format enables researchers to compare their results with the legacy of previous knowledge and supports bioinformatics tools and model building. Here, we summarize our progress with RegulonDB since our last Nucleic Acids Research publication describing RegulonDB, in 2013. In addition to maintaining curation up-to-date, we report a collection of 232 interactions with small RNAs affecting 192 genes, and the complete repertoire of 189 Elementary Genetic Sensory-Response units (GENSOR units), integrating the signal, regulatory interactions, and metabolic pathways they govern. These additions represent major progress to a higher level of understanding of regulated processes. We have updated the computationally predicted transcription factors, which total 304 (184 with experimental evidence and 120 from computational predictions); we updated our position-weight matrices and have included tools for clustering them in evolutionary families. We describe our semiautomatic strategy to accelerate curation, including datasets from high-throughput experiments, a novel coexpression distance to search for `neighborhood' genes to known operons and regulons, and computational developments